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Determination of β-Carotene in Spirulina by RP-HPLC

LUO Guang-hong，WANG Qin，YUAN Xiao-long，ZU Ting-xun   

  1. 1. Kaiyuan Bio-tech Development Center, Microalgae Engineering Research Center of Gansu Province, Hexi University,Zhangye 734000, China；2. Zhangye Institute for Food and Drug Control, Zhangye 734000, China；
    3. Zhangye Medical College, Zhangye 734000, China
  • Online:2013-11-25 Published:2013-12-05

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Abstract:

Objective: To establish a method for the determination of β-carotene in Spirulina using RP-HPLC. Methods:
A Hypersil ODS-2 C18 (150 mm×4.6 mm, 5 μm) was used for the analysis with methanol-acetonitrile (90:10, V/V) as the
mobile phase at a flow rate of 1.2 mL/min at 30℃. The detection wavelength was 448 nm. Results: The linear regression
equation for β-carotene was Y = 1388.33X + 17.74 (r = 0.9999), which was linear over the range of 0.517–4.653 μg, and the
average recovery rate was 98.03% with RSD of 0.94% (n = 6). Conclusion: This method is accurate and repeatable, and can
provide a scientific basis for the quality standardization of Spirulina products.

Key words: RP-HPLC；Spirulina；&beta, -carotene；content