Establishment and Application of Fluorescent Real-Time PCR for the Detection of Duck Meat in Foods

doi:10.7506/spkx1002-6630-201324019

Abstract

Abstract:

Fluorescent real-time PCR system for the assay of duck meat in foods was established on the basis of a pair of
primers and the TaqMan probe specific for duck was designed according to the sequence of duck nuclear gene (IL-2). We
also designed and established an internal amplification control (IAC) and TaqMan probe for IAC. Results showed that the
specificity of TaqMan real-time PCR was reliable and it could detect the presence of duck meat even when the concentration
of DNA was reduced to 0.5 ng. The sensitivity of this method was 0.1%. Its accuracy was verified with 38 food samples
from the market.

Key words: nuclear DNA, internal amplification control, fluorescent real-time polymerase chain reaction, duck meat

CHENG Xin，HE Wei-ling，HUANG Ming. Establishment and Application of Fluorescent Real-Time PCR for the Detection of Duck Meat in Foods[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201324019.

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Establishment and Application of Fluorescent Real-Time PCR for the Detection of Duck Meat in Foods

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