Isolation and purification and detection of anthraquinone compounds from the root of Actinidia arguta Sieb. et Zucc.

Abstract

Abstract: Abstract: Isolation and purification of anthraquinone from the root of Actinidia arguta Sieb. et Zucc by polyamide column chromatography and silica column chromatography. Determination of the content of anthraquinone compounds in the root of Actinidia arguta Sieb. et Zucc by high performance liquid chromatography. The results show that the optimum isolation conditions are found as follows：the type of silica gel was silica gel G-200, the type of chromatographic column wasφ1.1×100, the eluent speed of flow was 1.5ml/min. The content of main anthraquinone fractions composed of emodin and chrysophanol its contents were: 46μg / g and 157.5 μg / g from the root of Actinidia arguta Sieb. et Zucc. was determined by methanol: water = 90:10 as mobile phase, 254 nm as detecting wavelength of high performance liquid chromatograph.

Key words: Key words：root of Actinidia arguta Sieb. et Zucc, anthraquinone, isolation and purification, detection

CLC Number:

TS255.1

References

[1]翟延君,冯夏红,康廷国,等.软枣猕猴桃根的生药鉴定[J].中药材, 1993,16(3):19-21.
[2]侯方玉,孙延搏,陈飞,等.长白山产软枣猕猴桃茎多糖免疫药理作用的研究[J].中国中药杂志,1995,20(1):42-44.
[3]侯芳玉,陈飞,陆意,等.长白山产软枣猕猴桃茎多糖抗感染和抗肿瘤作用的研究[J].白求恩医科大学学报,1995,21(5):472-475.
[4]祝德秋,刘中申.软枣猕猴桃抗衰老作用的研究[J].湖南医学院学报,1996,2(2):54-57.
[5]李继海,沈育杰,丰利.软枣猕猴桃复合保健饮料功能评价研究[J].特产研究,1999(3):22-24,38. [6]江苏新医学院.中药大辞典(下册) [M].上海:上海人民出版社, 2005
[7] 崔剑，李兆陇，洪啸吟. 蒽醌衍生物与β-胡萝卜素自由基抗氧化协同作用的研究[J]. 化学学报，2004，62（12）：1095-1l00.
[8] Yen GC，Duh， PD， Chuang， DY. Antioxidant activjty ofanthraquinones and anthrone[J]. Food Chemistry，2000，70 （4）：437-441.
[9] Yen GC，Chen HW，Duh PD. Extration and identification ofan antioxidative component from Jue ming zi（Cassia tora L.）[J]. Agriculture and Food Chemistry，1998，46：820-824.
[10] 吴琳.薄层层析法分析几种中药中蒽醌类成分.国际医药卫生导报,2004,10(22):82.
[11]周建青,郭珍,李林. 芦荟中蒽醌类物质的提取研究[J]. 安徽农业科学杂志, 2007,35(22):6707,6736.
[12]崔红花,赵英日,王淑美. 大黄蒽醌类成分的提取方法筛选及均匀设计优化工艺研究.中国药房. 2010,21(7):612-614.
[13]张福平,郑绵青,吴美娟. 革命菜黄酮类化合物提取及其抗氧化性研究[J].食品科学，2012,33(22):48-52
[14]张腾霄,王斌,马松燕.大黄游离蒽醌提取工艺的正交优化研究.人参研究杂志,2009,4:17-20.
[15]张春复,申野,段大航. 虎杖中游离蒽醌类化合物提取工艺条件优化研究[J].中国现代药物应用,2010,4(13):144-145
[16]邵信儒,孙海涛,刘颖.超声提取野生软枣猕猴桃多糖工艺优化[J].食品科学, 2012, 33(14):64-68
[17]朱培芳,赵荣华,施扬宪. HPLC法同时测定何首乌中二苯乙烯苷和5种蒽醌类化合物的方法[J].中华中医药杂志，2012,27(2):463-465
[18]刘三康.超临界流体萃取.HPLC法测定何首乌中大黄酸、大黄素及大黄素甲醚的含量.中草药,19991,30(4):258-260.
[19]靳丹虹,梁芳慧,李敬筠.微波辅助提取-HPLC测定决明子中的5种蒽醌类化合物.分子科学学报,2007,23(6):429-433
[20]魏玉辉, 武新安，陈岚．大黄蒽醌类成分含量测定方法实验研究[J].兰卅大学学报：医学版，2005，31(3):13—15.