Interactions of Bovine Serum Albumin with Lemon Yellow and Sunset Yellow Studied by Fluorescence Spectroscopy

doi:10.7506/spkx1002-6630-201409026

Abstract

Abstract:

Interactions between bovine serum albumin (BSA) and lemon yellow or sunset yellow were studied by UV-visible
absorption spectroscopy and fluorescence emission spectroscopy. The dynamic quenching rate constants at 310 K were
calculated according to Stern-Volmer equation to be 2.51 × 105 and 1.42 × 105 L/mol for lemon yellow and sunset yellow,
respectively. The static quenching constants at 310 K were calculated according to Lineweaver-Burk double-reciprocal
equation to be 5.79 × 105 and 5.56 × 105 L/mol, respectively. The number of binding sites at 310 K was calculated
according to lg((F0－F)/F)=lgK0+nlgρ to be 0.556 95 and 0.640 56, respectively. A good linear relationship was observed
between lemon yellow or sunset yellow concentration in the range of 0 – 3 μg/mL and fluorescence quenching intensity.

Key words: bovine serum albumin, lemon yellow, sunset yellow, fluorescence quenching

LIU Zhi-dong, HAN De-quan, YANG Wei-wei, LI Xue-ru, SHAO Shu-shuang, SUN Qing-shen. Interactions of Bovine Serum Albumin with Lemon Yellow and Sunset Yellow Studied by Fluorescence Spectroscopy[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201409026.

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Interactions of Bovine Serum Albumin with Lemon Yellow and Sunset Yellow Studied by Fluorescence Spectroscopy

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