Abstract:

A methodology regarding certification of certified reference material (CRM) of alliin was presented. The CRM
was prepared from fresh garlic by a series of extraction, separation and purification. The purity of the CRM was determined
by area normalization method. For the analysis of homogeneity, 10 bottles of samples were randomly taken. The results
were validated by F-test statistical method and showed that the homogeneity of samples was good. The results of longterm
stability for alliin were validated by t-test statistical method and indicated that the storage duration for alliin at 4 ℃
was 43 months. A cooperative certification test was conducted by 9 laboratories. The property value was determined to be
(99.49 ± 0.95)%. The CRM has passed the evaluation made by the Standardization Administration of the People’s Republic
of China and has been applied in the actual detection.

Key words: alliin, certified reference material, homogeneity, stability, certification