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Safety Assessment of Enterococcus Isolated from Mare’s Milk and Its Products

WANG Meng-jiao, LI Shao-ying, LI Shu-fen, HE Wen-ying, SONG Xiao-min, MA Chun-yan, LI Zhen, LUO Yuan   

  1. 1. College of Food Science and Engineering, Inner Mongolia Agricultural University, Hohhot 010018, China; 2. College of Life
    Sciences, Inner Mongolia Agricultural University, Hohhot 010018, China; 3. College of Science, Inner Mongolia Agricultural
    University, Hohhot 010018, China; 4. Huhhot Railway Administration, Hohhot 010057, China
  • Online:2014-09-15 Published:2014-09-12

Abstract:

Objective: This study aimed to isolate lactic acid bacteria (LAB) from mare’s milk and its products, which have
nationality characteristics and special functions. The mare’s milk and its products were made by natural fermentation in
pastoral areas of Inner Mongolia. In order to ensure their safe application in the food fermentation industry, it is necessary
to assess the safety of LAB isolated from mare’s milk and koumiss. Methods: Indole test, nitrate reductase assay, amino
decarboxylase test, hemolysis test, D-lactic acid assay and plasmid extraction were performed to identify whether harmful
metabolites or drug resistance exists in the tested strains. Results: Negative results were observed in all tests except for D-lactic
acid, which was detected in small amounts in strains JHZ9, JHZ15, JHZ25 and JNN1. Neither resistance to the tested drugs
nor transferable resistant plasmid was detected in seven tested strains. Conclusion: Neither harmful metabolites nor drug
resistant plasmids has been detected in the seven strains, which belong to Enterococcus.

Key words: lactic acid bacteria, safety, harmful metabolites, drug resistance, plasmids

CLC Number: