Abstract:

Fusarium graminearum macroconidia were quantitatively inoculated in rice culture, and the crude extracts of the
toxins were prepared and subjected to silica gel column chromatography. Deoxynivalenol (DON) and acetyldeoxynivalenol
(AcDON) isolates were monitored by thin-layer chromatography and the fractions rich in these toxins were pooled and
concentrated, respectively. Both fractions yielded a crystalline material through a simple crystallization step. The two
compounds were identified and confirmed by infrared spectroscopy, liquid chromatography-mass spectrometry and
1H NMR analysis in comparison to the reference standards or the published data. The liquid chromatography analysis
showed that the purity of the two toxins were both greater than 98%. This method developed here has overcome the disadvantages
of the conventional cumbersome multi-step procedure, and provided a simplified and efficient strategy suitable for large-scale
production and purification of the mycotoxin DON and its acetylated products AcDON from Fusarium graminearum rice cultures.

Key words: Fusarium graminearum, deoxynivalenol, acetyldeoxynivalenol, culture, preparation