Abstract:

This paper reported the preparation of the type Ⅱ collagen from chicken articular cartilage. Guanidine
hydrochloride and NaCl were used to remove the contaminating proteins, and the collagen was then digested with pepsin.
The study investigated effects of reaction temperature, reaction time and concentration of enzyme on extraction efficiency.
The results showed that guanidine hydrochloride at 4 mol/L removed heteropolysaccharide effectively. The optimal
conditions for collagen type Ⅱ extraction were as follows: temperature, 18 ℃; gastric protein concentration, 1.59%; and
digestion time, 43 h. Under these conditions, the extraction efficiency of collagen was 56.54%.

Key words: chicken stemal cartilage, type Ⅱ collagen, extract