Isolation and Purification of Antioxidant Components in Fruiting Body of Cordyceps militaris

doi:10.7506/spkx1002-6630-201508032

Abstract

Abstract:

The 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity was used as an indicator and the bioassayguided
fractionation was developed to isolate antioxidant components from the fruiting body of Cordyceps militaris. The
methanol extract of the fruiting body of Cordyceps militaris was separated by silica gel column chromatography and a
higher active antioxidant component CM9 was obtained. By using reversed-phase high performance liquid chromatographic
(RP-HPLC) method, CM9-1 was purified from component CM9 and was confirmed as a pure compound by thin layer
chromatography (TLC) and HPLC. The results of mass spectrographic analysis showed that molecular weight of the purified
antioxidant compound CM9-1 was 430 with a molecular formula of C22H22O9. At the concentration of 1.0 mg/mL, CM9-1
could decrease (73.67 ± 0.29)% of 0.4 mg/mL DPPH radicals. The results of this study could offer a new way to develop
health natural antioxidant.

Key words: Cordyceps militaris, fruiting body, antioxidant components, isolation, purification

CLC Number:

Q939.9

SHAO Ying, CHEN Anhui, ZHANG Chuanli, ZHOU YangSHAO Ying, CHEN Anhui, ZHANG Chuanli, ZHOU Yang. Isolation and Purification of Antioxidant Components in Fruiting Body of Cordyceps militaris[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201508032.

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Isolation and Purification of Antioxidant Components in Fruiting Body of Cordyceps militaris

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