Abstract:

In recent years, with the further functional study on the characteristics and applications of lactic acid
bacteria, molecular biology of lactic acid bacteria is also of concern. Cloning and functional expression of lactic acid bacteria
using molecular tools have become the latest hotspot. Researchers have found or built many gene expression vectors with
different characteristics which lay the foundation for gene cloning and expression of lactic acid bacteria. Based on the
characteristics of lactic acid bacteria, this paper summarizes the vectors which have been reported from the perspective of
safety. And we also review the applications of gene expression vectors for the production of polysaccharides, enzyme and
vaccine preparation, etc.

Key words: lactic acid bacteria, non-food-grade genetic expression vector, food-grade genetic expression vector, application