Abstract:

The epibiotic lactic acid bacteria (LAB) on the surface of organic radish were investigated by MRS culture,
physiological and biochemical characteristics, RAPD, 16S rRNA, antibiotic resistance and phenotype polymorphism.
Approximately 4.67 × 103 CFU/cm2 LAB including 187 isolates colonized on the surface of organic white radish. These
isolates were assigned to Pediococcus pentosaceus (114/187), Weissella cibaria (9/187), Leuconostoc mesenteroides
(18/187), Leuconostoc pseudomesenteroides (7/187), Leuconostoc citreum (20/187) and Leuconostoc holzapfelii (19/187),
respectively. And 25 (13.37%) isolates displayed single-drug or multi-drug resistance to tetracycline (TET), streptomycin
(STR) and penicillin G (PEN). Of these P. pentosaceus isolates, 2 had triplicate resistance to STR, TET and PEN,
13 had double resistance to TET and STR, and 4 had single resistance to STR. Of these L. citreum isolates, 1 had triplicate
resistance to STR, TET and PEN, and 1 had the solo resistance to STR. Furthermore, 2 L. mesenteroides isolates with
TET resistance and double resistance TET and PEN respectively were found, and on the surface of white radish there were
1 L. pseudomesenteroides strain and 1 W. cibaria strain showing double resistance to STR and TET and double resistance to
STR and PEN, respectively.

Key words: organic white radish, lactic acid bacteria, tetracycline, streptomycin, penicillin G, antibiotic resistance