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Advances in Research on Milk-Clotting Enzymes

HANG Feng1,2, HONG Qing2, WANG Qinbo2, LIU Peiyi2, LIU Zhenmin2, CHEN Wei1,*   

  1. 1. State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University,
    Wuxi 214122, China; 2. State Key Laboratory of Dairy Biotechnology, Dairy Research Institute,
    Bright Dairy and Food Co. Ltd., Shanghai 200436, China
  • Online:2016-02-15 Published:2016-02-26

Abstract:

Calf rennet is conventionally used as milk coagulant for the production of cheese. However, the supply of
calf rennet is not equivalent to the demand in cheese industry, which merely meets 20%–30% of the global demand for
cheese production. Due to the scarcity and high price of calf rennet, it is necessary and urgent to find potential substitutes.
This review gives an overview of the current discoveries of calf rennet, the characteristics of different types of milkclotting
enzymes including animal rennet, recombinant chymosin, plant- and microbial-derived coagulants, and specifies
the application of coagulants in cheese production. The objective of this review is to provide the fundamental theory and
inspiring ideas for the researchers and manufacturers to find calf rennet substitutes.

Key words: chymosin, milk-clotting activity, proteolytic activity, cheese

CLC Number: