Determination of the Protein Content of Milk by Digital Imaging Colorimetry

doi:10.7506/spkx1002-6630-201604027

Abstract

Abstract:

The grey value obtained by digital imaging for the blue complex formed from the binding interaction of
Coomassie brilliant blue with proteins is positively linearly related to the concentration of proteins. Thus, a method was
established for the determination of the protein content of milk by digital imaging colorimetry. The imaging conditions,
color development conditions and interfering substances were investigated. The recovery rates for spiked milk samples were
between 97.5% and 102.6% with a relative standard deviation (RSD) less than 2%. Consistent results were obtained using
digital imaging colorimetry and spectrophotometry, but digital imaging colorimetry was more convenient and faster.

Key words: digital imaging colorimetry, protein, Coomassie brilliant blue

CLC Number:

TS207.3

YUE Wei, LIU Le. Determination of the Protein Content of Milk by Digital Imaging Colorimetry[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201604027.

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Determination of the Protein Content of Milk by Digital Imaging Colorimetry

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