Abstract:

In order to compare extraction efficiencies of total protein from the yeast-like conidia of Tremella fuciformis by
Tris-saturated phenol method, trichloroacetic acid (TCA)-acetone precipitation method and their combination, the cells were
disrupted by ultrasound and high-pressure homogenization, the efficiency of cell disruption was observed by microscope,
protein content was determined by the Bradford method, and SDS-PAGE gel electrophoresis analysis was performed. The
results showed that the efficiency of cell disruption by high pressure homogenization was better than that by ultrasound. The
protein contents obtained by three extraction methods were 2.33, 1.26 and 1.02 mg/mL, respectively. The brightness and
resolution of electrophoresis bands with traditional coomassie brilliant blue staining by Tris-saturated phenol method were
better than those obtained by two other methods. Hence Tris-saturated phenol method can be considered as a feasible method
for protein extraction from the yeast-like conidia of T. fuciformis.

Key words: Tremella fuciformis, yeast-like conidia, protein extraction method