Development of using ELISA and UPLC-MS detection of zearalenone form corn

Abstract

Abstract: A ELISA and Ultra Performance Liquid Chromatograph-Mass-Mass(UPLC-MS-MS) spectrometry method was developed for the determination of zearalenone in corn samples. Samples were extracted with acetonitrile-water-acetic acid(70:29:1) and then determined by ELISA and UPLC-MS-MS. In the study, special attention was given to the stability of the system and the matrix effects frequently observed in the process. The recovery purification problem for UPLC-MS-MS sample was also critically studied. Proper approaches dealing with those problems were obtained. It demonstrated that our analysis method is easy and fast, which could meet the requirements for determination of large amount samples.

Key words: Zearalenone, ELISA, UPLC-MS-MS, Grain, Detection

CLC Number:

TS252

Yan LI Shi-Chun PEI Yu-Ping ZHEN. Development of using ELISA and UPLC-MS detection of zearalenone form corn[J]. FOOD SCIENCE, 0, (): 0-0.

References

[1]张宇昊，杨琳，马良.真菌毒素同时检测方法研究进展[J].中国粮油学报, 2011, 26(6):123-128 [2]曾红燕，黎源倩，敬海泉.高效液相色谱法测定粮食中玉米赤霉烯酮及其代谢物[J].分析化学研究简报, 2006, 34(3):351-354 [3]RAHMANI A, JINAP S, SOLEIMANY F.Validation of the procedure for the simultaneous determination of aflatoxins ochratoxin A and zearalenone in cereals using HPLC-FLD[J].Food Additives and Contaminants, 2010, 27(12):1683-1693 [4]OFITSEROVA M, NERKAR S, PICKERING M, et al.Multiresidue mycotoxin analysis in corn grain by column high-performance liquid chromatography with postcolumn photochemical and chemical derivatization: single-laboratory validation[J].Journal of AOAC International, 2009, 92(1):15-26 [5]IBá?EZ-VEA M, CORCUERA L A, REMIRO R, et al.Validation of a UHPLC-FLD method for the simultaneous quantification of aflatoxins,ochratoxin A and zearalenone in barley[J].Food Chemistry, 2011, 127(1):351-358 [6]WANG Yaling, CHAI Tongjie, LU Guozhong, et al.Simultaneous detection of airborne aflatoxin,ochratoxin and zearalenone in a poultry house by immunoaffinity clean-up and high-performance liquid chromatography[J].Environmental research, 2008, 107(2):139-144 [7]周晓，谢体三，刘运龙.技术在食品真菌毒素检测中的应用[J].粮食与食品工业, 2007, 14(5):49-52 [8]应永飞，朱聪英，韦敏珏，等.液相色谱-串联质谱法测定饲料中14种霉菌毒素及其类似物[J].分析化学研究报告, 2010, 38(12):1759-1764 [9]胡骁飞，职爱民，刘庆堂，等.真菌毒素检测方法新进展[J].中国农学通报, 2010, 26(8):100-103 [10]宫小明，任一平，董静，等.超高效液相色谱串联质谱法测定花生粮油中种真菌毒素[J].分析测试学报, 2011, 30(1):6-12 [11]廉慧锋，赵笑天，王蓉珍，等.超高效液相色谱-串联质谱法同时测定玉米、花生、麦仁中的种真菌毒素[J].食品科学, 2010, 31(20):360-366 [12]杨万颖, 郑彦婕, 李碧芳, 等.液相色谱串联质谱法同时测定小麦粉中种真菌毒素[J].广东化工, 2008, 35(9):123-128 [13]张园园，裴世春，才琳，等.抗黄曲霉毒素M1单克隆抗体的制备及定量ELISA方法的建立[J].中国预防兽医学报, 2008, 30(10):800-804 [14]马智鸿，黄飚，屠蔷，等.应用生物素-链霉亲和素的酶联免疫吸附法检测谷物中的玉米赤霉烯酮[J].安徽农业科学, 2009, 37(15):6849-6851 [15]MAKIAíNNE ERBS, NICCOLO HARTMANN, THOMAS D.BUCHELI[J].Determination of the Cross-Reactivities for α-Zearalenol, β-Zearalenol, Zearalanone, α-Zearalanoi, and β-Zearalanol on Three Commercial Immunoaffínity Columns Targeting Zearalenone.JOURNAL OF AOAC INTERNMIONAL VOL, 2007, 90(4):1197-1202 [16]齐美玲.液相色谱一质谱法在生物样品药物定量分析中的基质效应[J].药物分析杂志, 2005, 25(4):476-479 [17]JANSSON C, PIHLSTROM T, OSTERDAHL B G, et al.A new multi-residue method for analysis of pesticide residues in fruit and vegetables using liquid chromatography with tandem mass spectrometric detection[J].Journal of Chromatography A, 2004, 1023(1):93-104 [18]HERNANDEZ F, SANCHO J V, POZO O J.Critical review of the application of liquid chromatography/mass spectrometry to the determination of pesticide residues in biological samples[J].Analytical and Bio-analytical Chemistry, 2005, 382(4):934-946 [19]ZROSTLIKOVA J, HAJSLOVA J, POUSTKA J, et al.Alternative calibration approaches to compensate the effect of co-extracted matrix components in liquid chromatography-electrospray ionisation tandem mass spectrometry analysis of pesticide residues in plant materials[J].Journal of Chromatography A, 2002, 937(1-2):13-26 [20]BESTER K, BORDIN G, RODRIGUEZ A, et al.How to overcome matrix effects in the determination of pesticidesin fruit by HPLC-ESI-MS-MS[J].Fresenius Journal of Chemistry, 2001, 371(10):550-555