Abstract: In order to analyze the formation kinetics of fluorescent advanced glycation end products (AGEs), this work
addressed the effects of bovine serum albumin (BSA) concentration, fructose concentration, temperature, and pH on the
formation of fluorescent AGEs in a simulated processing system consisting of BSA and glucose. Fluorescence intensity was
used to measure the fluorescent AGEs content. The kinetics of substrate depletion was also investigated. The results showed
that the fluorescent AGEs content increased with increasing BSA concentration, fructose concentration, temperature and pH.
After 24 min reaction, fructose and amino acid concentrations decreased by 1.25 × 10-2 and 1.92 × 10-4 mol/L, respectively,
indicating a larger decrease in fructose concentration. The formation of fluorescent AGEs followed zero-order kinetics,
with a maximum rate constant of 31.57 AU/min and an activation energy of 70.58 kJ/mol, and substrate depletion followed
first-order kinetics.

Key words: advanced glycation end products (AGEs), bovine serum albumin/fructose system, kinetics, reaction order