Abstract: In order to evaluate the antioxidant activity and components of spine grape peel, crude extract from spine grape peel was prepared by ultrasonic assisted extraction with acidified methanol as the extraction solvent and separated by macroporous resin adsorption to obtain five main fractions (I–V). Spine grape peel showed high contents of total phenolics (1.836 mg/g mf), total flavonoids (0.874 mg/g mf), VC (3.567 mg/g mf), total condensed tannins (3.578 mg/g mf) and total anthocyanins (2.970 mg/g mf) . The antioxidant activity of the methanol extract as evaluated by means of 1,1-diphenyl- 2-picrylhydrazyl (DPPH) assay, 2,2’-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid (ABTS) assay and ferric reducing antioxidant power (FRAP) was 22.325, 17.595 and 26.487 μmol TE/g mf, respectively. Among 5 fractions, the watereluted fraction I contained the highest contents of phenolics, flavonoids, VC, tannin and anthocyanins (0.876, 0.116, 1.577, 1.576 and 1.330 mg/g mf, respectively) and presented the best antioxidant activity (3.636, 10.109 and 13.415 μmol TE/g mf for DPPH radical scavenging activity, ABTS+· scavenging activity and FRAP, respectively). Significant correlations were observed between antioxidant activity and anthocyanins, tannin and VC contents, which suggested that these compounds might be the major antioxidants contributing to the observed activity. Therefore, spine grape peel is a promising source of antioxidants and can be used as a dietary supplement for health promotion.

Key words: spine grape peel, antioxidant activity, active components