FOOD SCIENCE ›› 2011, Vol. 32 ›› Issue (9): 170-173.doi: 10.7506/spkx1002-6630-201109039

• Bioengineering • Previous Articles     Next Articles

Comparison of Four Methods for Genomic DNA Extraction from Yeasts

ZHAO Hong-yu1,2，LI Jun1,2，ZHAO Yue1，WANG Xin1，CAI Lu1,2,*   

  1. 1. School of Mathematics, Physics and Biological Engineering, Inner Mongolia University of Science and Technology, Baotou 014010, China；2. Institute of Bioengineering and Technology, Inner Mongolia University of Science and Technology, Baotou 014010, China
  • Online:2011-05-15 Published:2011-04-11

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Abstract: In order to screen a simple and effective method for the extraction of yeast genomic DNA, different methods, such as lysozyme digestion, overnight snailase digestion, snailase digestion followed by repeated freezing and thawing and grinding with glass beads were compared for their performance based on cell disruption observed under inverted fluorescence microscope, DNA concentration and purity determined using UV spectrophotometer and DNA quality detected by agarose gel electrophoresis and PCR. Except lysozyme digestion, all other methods provided high-quality yeast genomic DNA. The method of grinding with glass beads presented the advantages of high quality extracts, lower cost, less time consuming and ease of operation.

Key words: yeast, genomic DNA, grinding with glass beads, snail enzyme, lysozyme

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