Comparison of Different Cell Wall Disruption Methods for Yeast Total RNA Extraction

doi:10.7506/spkx1002-6630-201111035

FOOD SCIENCE ›› 2011, Vol. 32 ›› Issue (11): 161-164.doi: 10.7506/spkx1002-6630-201111035

• Bioengineering • Previous Articles Next Articles

Comparison of Different Cell Wall Disruption Methods for Yeast Total RNA Extraction

YI Yi1，RONG Yuan-ping1，CHENG Qian-wei1，LI Ya1，WANG Xiao-lin2

1. Department of Biological and Chemical Engineering, Guangxi University of Technology, Liuzhou 545006, China；2. State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences of the PLA, Beijing 100071, China

Online:2011-06-15 Published:2011-05-13

Abstract

Abstract: Five methods such as liquid nitrogen grinding, repeated freeze-thawing, ultrasonic treatment, vortex shaking with glass beads and snailase hydrolysis were used to break the cell wall of yeast for the extraction of total RNA by the Trizol method. Based on total RNA concentration and purity, these methods were compared for their effects on total RNA extraction from yeast. The results showed liquid nitrogen grinding and repeated freeze-thawing were the most effective and convenient methods for total RNA extraction from yeast by Trizol.

Key words: Trizol, RNA extraction, yeast, cell wall disruption

CLC Number:

Q936

YI Yi，RONG Yuan-ping，CHENG Qian-wei，LI Ya，WANG Xiao-lin. Comparison of Different Cell Wall Disruption Methods for Yeast Total RNA Extraction[J]. FOOD SCIENCE, 2011, 32(11): 161-164.

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Comparison of Different Cell Wall Disruption Methods for Yeast Total RNA Extraction

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