Abstract: In order to achieve maximum degreasing rate, the hydrolysis of cultured large yellow croaker fillets by alkaline lipase was optimized by the combined use of single factor and orthogonal array design methods. The optimum hydrolysis conditions for degreasing large yellow croaker fillets were temperature 32 ℃, reaction pH 8.5, enzyme concentration 60 U/mL, reaction time 60 min, substrate-to-enzyme solution ratio 1:4 (by mass), respectively. Under these conditions, the degreasing rate was 67.32%.

Key words: cultured large yellow croaker (Pseudosciaena crocea), alkaline lipase, degreasing rate