Macroporous Resin Adsorption for Purification of Total Flavonoids from Actinidia arguta Fruits

doi:10.7506/spkx1002-6630-201112032

Abstract

Abstract: In the present study, macroporous resin adsorption was used to probe the purification of total flavonoids from the crude extract of Actinidia arguta fruits, prepared by ultrasonic-assisted ethanol and reextraction with petroleum ether. Among the nine macroporous resins investigated, HPD 600 exhibited the best performance in adsorbing and desorbing total flavonoids from Actinidia arguta fruits and its optimum working parameters were sample solution pH 3－4, sample concentration of 0.5 mg/mL, sample loading amount of 3 BV, washing water amount of 3 BV, 4 BV of 80% ethanol as the eluent at a flow rate of 2 mL/min. After the purification, the average recovery rate and purity of total flavonoids was 86.5% and 37.2%, respectively. Therefore, HPD600 is suitable for the purification of total flavonoids from Actinidia arguta fruits.

Key words: macroporous resin, Actinidia arguta, flavonoids, purification

CLC Number:

S663.4

LIU Chang-jiang，LUAN Yun-feng，WANG Fei，LIANG Shuang，YANG Zhi-Chao. Macroporous Resin Adsorption for Purification of Total Flavonoids from Actinidia arguta Fruits[J]. FOOD SCIENCE, 2011, 32(12): 145-149.

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Macroporous Resin Adsorption for Purification of Total Flavonoids from Actinidia arguta Fruits

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