Abstract: In order to establish a method for determining triadimenol residues in banana by ultra-performance liquid-chromatography-mass spectrometry (UPLC-MS/MS), the triadimenol residues in samples were extracted with acetonitrile, cleaned up ony Florisil SPE column, separated on C18 reverse phase column using methanol-0.05% formic acid as mobile phase, and detected with positive ionization electrospray ionization-mass spectrometry under a multiple reaction monitoring (MRM) mode. An excellent linear relationship between triadimenol concentration in the range of 0－0.16 mg/L and peak area was achieved with a relative standard deviation of 4.8%－10%. The detection limit for triadimenol in banana was 1.0μg/mL and recovery rate of this method was in the range of 87.8%－97.8% with a relative standard deviation of 4.8%－10%. This developed method is simple, rapid, sensitive and can therefore be used for the determination of triadimenol in banana.

Key words: ultra-performance liquid-chromatography-mass spectrometry (UPLC-MS/MS), banana, triadimenol