Genomic DNA Extraction from Rat Faecal and Intestinal Microflora Based on DGGE Analysis

doi:10.7506/spkx1002-6630-201117044

Abstract

Abstract: In this study, SD rats were fed dietary fiber from tomato skin for understanding the effect of dietary fiber on intestinal flora. The feces and cecum of rats were collected and stored at －70 ℃. Bacterial genomic DNA was extracted from feces and cecum using TIANamp bacterial DNA kit, proteinase K-CTAB (cetyltrimethylammonium bromide) and muramidase protocol, respectively. The extracted DNA was assayed by agarose gel electrophoresis, bacterial universal primer PCR and denaturing gradient gel electrophoresis (DGGE). Results showed that more and better DNA was extracted by using modified muramidase method with lower cost and shorter extraction time when compared with other methods. Therefore, the modified muramidase protocol is the best for DNA extraction from feces and cecum of rats.

Key words: DNA extraction, rat, faecal, intestinal microflora, denaturing gradient gel electrophoresis (DGGE)

CLC Number:

Q523

ZHENG Gang，CHEN Ji-ren，HU Bo-wen，CHEN Yan-bin，ZHAO Guo-hua,. Genomic DNA Extraction from Rat Faecal and Intestinal Microflora Based on DGGE Analysis[J]. FOOD SCIENCE, 2011, 32(17): 215-218.

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Genomic DNA Extraction from Rat Faecal and Intestinal Microflora Based on DGGE Analysis

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