Abstract: Akkermansia muciniphila is a strictly anaerobic gram-negative bacterium that can specifically degrade mucin in the mucus layer of the gastrointestinal tract. It was first isolated from human feces. A. muciniphila plays an important role in promoting host health at the mucosal interface, which has recently become a hot spot in the field of intestinal microecology and probiotics. This paper is focused on summarizing the current state of the art in the genomics, molecular biological identification and typing, and isolation and purification of A. muciniphila. The merits and drawbacks of the existing culture conditions as well as the existing methods for the isolation and purification of A. muciniphila. In addition, we compare the colonization characteristics in different populations of A. muciniphila. The aim of this review is to analyze the possibility of microbiota transplantation, extend the study of the molecular mechanism of action of A. muciniphila and thereby develop new probiotic products.

Key words: Akkermansia muciniphila, isolation, colonization, intestinal microbiome