Preparation of Enzyme-Labeled Methamidophos for Application in Enzyme Thermal Sensor

doi:10.7506/spkx1002-6630-201206012

FOOD SCIENCE ›› 2012, Vol. 33 ›› Issue (6): 54-58.doi: 10.7506/spkx1002-6630-201206012

• Processing Technology • Previous Articles Next Articles

Preparation of Enzyme-Labeled Methamidophos for Application in Enzyme Thermal Sensor

Nguyen Van-luc，YU Jin-song，XU Fei*，HUA Ze-zhao

(Institute of Food Safety, University of Shanghai for Science and Technology, Shanghai 200093, China)

Online:2012-03-25 Published:2012-03-03

Abstract

Abstract: The preparation of enzyme-labeled methamidophos was carried out by carbodiimide (EDC) method. Based on the effect on the remaining amount of methamidophos, the optimal ratio of methamidophos to succinic anhydride was determined to be 1:1.5 and the optimal reaction time 15 h. With increasing ratio between methamidophos derivatives and glucose oxidase, the coupling rate of enzyme-labeled methamidophos increased, while the enzyme activity decreased. Considering that enzyme-labeled pesticides need sufficient esterase inhibitory activity, the ratio of methamidophos derivatives to glucose oxidase was determined as 10:1.

Key words: methamidophos derivative, glucose oxidase (GOD), enzyme-labeled pesticides

CLC Number:

TS201.6

NguyenVan-luc，YUJin-song，XUFei，HUAZe-zhao. Preparation of Enzyme-Labeled Methamidophos for Application in Enzyme Thermal Sensor[J]. FOOD SCIENCE, 2012, 33(6): 54-58.

[1]	Yong Wu Hong-bing CHEN. Research progress of cashew nut allergy [J]. FOOD SCIENCE, 0, (): 0-0.
[2]	Xin-Yue HU. Research on Construction of Food Cold Chain Traceability System Based on Quantum Blockchain [J]. FOOD SCIENCE, 0, (): 0-0.
[3]	SHI Wen-Zheng Ying 无LU. Study on the reduction effect of Lactobacillus plantarum on the immunological activity of tropomyosin [J]. , 0, (): 0-0.
[4]	. Detection of Escherichia coli O157:H7 by Cascade Signal Transduction System Combined with Immunochromatography [J]. , 0, (): 0-0.
[5]	Jian Fei JianYANG ZUO Yong Jia XU. Rapid Detection reagent of nitrite and its application in pickles [J]. , 0, (): 0-0.
[6]	Fei JIA. An impedimetric aptasensor based on " rGO/CNT-AuNPs " nanocomposite for the detection of Pseudomonas aeruginosa [J]. , 0, (): 0-0.
[7]	Xiang YiLIU Yu MA Donghui Li Linglin Fu Liu Guang-Ming. Dietary structure and components affecting food allergy through regulation of intestinal microflora: A review [J]. , 0, (): 0-0.
[8]	晋豪 ZHANG Xing-Lian Xu. Research progress and trend of new characterization technology of fresh meat freshness:A Review [J]. FOOD SCIENCE, 0, (): 0-0.
[9]	. Analysis and consideration on the difference of risk factor limit in infant food safety standards between China and international organizations [J]. FOOD SCIENCE, 0, (): 0-0.
[10]	Hui ZHANG Rui-Li YANG Liu Fang Lu na. Effect of food-grade κ-carrageenan on the inducing-risk of colitis and accumulation of body fat in obese mice [J]. , 0, (): 0-0.
[11]	LI Linqiong, HONG Jing, ZHANG Lijun, GAO Yulong. Effect of Acid Stress on Acid Resistance, Cell Membrane and Membrane Protein of Salmonella typhimurium [J]. FOOD SCIENCE, 2021, 42(15): 27-36.
[12]	QU Jingyao, LIN Yucheng, MAO Xin, LU Guozhu, YUAN Zhipeng, ZHANG Yaoyao, YOU Yanli, LI Yanshen. Occurrence Patterns of Alternaria Toxins in Tomatoes Inoculated with Alternaria spp. [J]. FOOD SCIENCE, 2021, 42(15): 180-186.
[13]	XuBo ZHAO. Prediction Model Construction of Aspergillus flavus Growth and Toxin Accumulation In Chili Powder [J]. , 0, (): 0-0.
[14]	CHEN Yanfen. Migration of Mineral Oils Hydrocarbons from Fast Food Packaging Paper into Solid Food Simulant [J]. FOOD SCIENCE, 0, (): 0-0.
[15]	HUI BoWen Rui LIU. Assessment and Case Analysis of Food Carbon Footprint from the Perspective of Life Cycle [J]. FOOD SCIENCE, 0, (): 0-0.

Preparation of Enzyme-Labeled Methamidophos for Application in Enzyme Thermal Sensor

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments