FOOD SCIENCE ›› 2012, Vol. 33 ›› Issue (9): 154-157.doi: 10.7506/spkx1002-6630-201209032

• Bioengineering • Previous Articles     Next Articles

Comparison of Three Methods for Total DNA Extraction of Intestinal Microorganisms in Puffer Fish

LI Dan,PAN Ying-jie,ZHAO Yong,SUN Xiao-hong*   

  1. (Shanghai Engineering Research Center of Aquatic-Product Processing and Preservation, College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China)
  • Online:2012-05-15 Published:2012-05-07

Abstract: Objective: To establish an effective and economical method for total DNA extraction from intestinal contents in puffer fish. Methods: Three DNA extraction methods including phenol-chloroform-isoamyl alcohol extraction method, QIAamp DNA Stool Mini Kit, modified FastDNA SPIN Kit for soil were designed and used in combination with polymerase chain reaction-denatured gradient gel electrophoresis (PCR-DGGE) technique to analyze the microbial diversity in the intestine of puffer fish. Results: Much more DNA and higher microbial diversity were extracted by phenol-chloroform-isoamyl alcohol extraction method and QIAamp DNA Stool Mini Kit compared with modified FastDNA SPIN Kit for soil. Conclusion: Phenol-chloroform-isoamyl alcohol extraction method is an effective and economical method for DNA extraction, and can be applied for studying microbial diversity.

Key words: intestinal contents, DNA extraction, microbial diversity, polymerase chain reaction-denatured gradient gel electrophoresis (PCR-DGGE)

CLC Number: