Preparation of Blueberry Anthocyanin Extract by Ultrasound-Flash Method and Evaluation of Its Anti-Tumor Activity in Vivo and in Vitro

Abstract

Abstract: Ultrasonic-flash extraction (UFE) was employed to extract the anthocyanins from blueberry. The effect of ultrasonic power, flash extraction speed, extraction time, solid-to-liquid ratio and ethanol concentration on the yield of anthocyanins were investigated. Genetic algorithm is used to optimize the process parameters of extraction considering single factor experiment. In vitro MTT and Annexin V-FITC/PI double label staining were employed to investigate the effects of anthocyanins extracts at different concentrations on proliferation and apoptosis of S180 cells. In vivo antitumor activity of anthocyanins extracts from was tested by mouse S180 tumor-bearing mice model and ELISA. The results showed that the optimum extracting parameters to achieve the highest the yield of anthocyanins of 2.71±0.08 mg/g from blueberry was obtained under an extraction time of 10 min, ultrasonic power of 450 W, flash extraction speed of 8400 r/min, solid-to-liquid ratio of 1:30 g/mL and ethanol concentration of 68%. In vitro experiments showed that anthocyanins extracts could remarkably inhibit the proliferation of S180 cells, induce the apoptosis of S180 cells, and block the cells in S phase. In vivo anti-tumor test in tumor-bearing mice showed that the mass of tumors in anthocyanins extracts low-dose, medium-dose and high- dose groups was significantly lower than that in model control group (p<0.05) , and the anti-tumor rates were 26.36%, 33.33% and 46.51%, respectively. The levels of IL-12 and TNF-α in serum of mice bearing anthocyanins extracts low-dose, medium-dose and high- dose groups were markedly higher than those of model control group (p<0.05), while the level of IL-10 were significantly lower than that of model control group (p<0.05) . In conclusion, the UFE is an efficient method for anthocyanins extraction, and the anthocyanins extracts can inhibit the growth of tumors by inducing apoptosis and enhancing immunity.

Key words: Ultrasonic-flash extraction, blueberry, anthocyanins, process, anti-tumor activity

CLC Number:

TS255.42

xuehongkun. Preparation of Blueberry Anthocyanin Extract by Ultrasound-Flash Method and Evaluation of Its Anti-Tumor Activity in Vivo and in Vitro[J]. FOOD SCIENCE, 0, (): 0-0.

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