FOOD SCIENCE ›› 2020, Vol. 41 ›› Issue (9): 66-73.doi: 10.7506/spkx1002-6630-20190412-176

• Nutrition & Hygiene • Previous Articles     Next Articles

Intracellular Antioxidant Activity of Two Terpenoids in Baijiu

ZHANG Qian, ZHU Tingting, HUANG Mingquan, WEI Jinwang, WU Jihong, HUO Jiaying   

  1. (1. Beijing Laboratory of Food Quality and Safety, Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Key Laboratory of Flavor Chemistry, Beijing Technology and Business University, Beijing 100048, China; 2. Beijing Shunxin Agriculture Co. Ltd. Niulanshan Distillery, Beijing 101301, China)
  • Online:2020-05-15 Published:2020-05-15

Abstract: Headspace solid phase microextraction coupled with gas chromatography-mass spectrometry was applied for the qualification and quantification of terpenoids in 13 base Baijius and 3 commercial Baijius of Niulanshan “Erguotou”. Totally 5 terpenoids were identified by the external standard method, including β-caryophllene (BCP) and geranylacetone (GAT), which was first discovered in Erguotou Baijiu. The protective effects of GAT and BCP on oxidative damage induced by 2,2’-azobis(2-methylpropanimidamidine)dihydrochloride (AAPH) in HepG2 cells were investigated. The results showed that at the same concentration as in Erguotou, both GAT and BCP had a potent scavenging effect against intracellular reactive oxygen species by increasing the activity of intracellular antioxidant enzymes including catalase, superoxide dismutase and glutathione peroxidase and the level of non-enzymatic antioxidants such as glutathione (GSH). In addition, they could decrease the level of malondialdehyde (MDA), thereby enhancing cellular antioxidant defense capacity in a dose-dependent manner. This study provides fundamental data supporting the health benefits of Baijiu and the healthy development of the Baijiu industry.

Key words: Niulanshan Erguotou Baijiu, geranylacetone, β-caryophllene, headspace-solid phase microextraction, antioxidant activity

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