Abstract: This investigation aims to evaluate the in vitro anti-inflammatory effect of mung bean protein hydrolysate (MBPH). A mouse model of acute lung injury (ALI) was constructed by intranasal instillation of lipopolysaccharide (LPS), and the anti-inflammatory of MBPH was evaluated by assessing pulmonary histopathological changes, the ratio between wet mass and dry mass, myeloperoxidase (MPO) activity, and cytokine concentrations, protein concentration and neutrophil count in bronchoalveolar lavage fluid (BALF). The results showed that MBPH relieved lung injury in a dose-dependent manner, reduced the wet/dry mass ratio and MPO activity of lung tissue, and decreased the concentrations of proteins, pro-inflammatory cytokines and neutrophils in BALF. The anti-inflammatory effect of high-dose MBPH was similar to that of dexamethasone when administered at a dose of 5 mg/(kg mb · d). The above findings indicated that MBPH has an anti-inflammatory effect, which can exert its protective effect on ALI by reducing pulmonary edema and pulmonary microvascular leakage, improving the pathological changes of lung tissue, reducing neutrophil infiltration in BALF, and inhibiting the secretion of cytokines by inflammatory cells.

Key words: mung bean protein hydrolysate; lipopolysaccharide; acute lung injury