FOOD SCIENCE ›› 2020, Vol. 41 ›› Issue (21): 154-159.doi: 10.7506/spkx1002-6630-20191113-170

• Nutrition & Hygiene • Previous Articles     Next Articles

Protective Effect of Polyphenols Extracted from Honey against Acute Alcoholic Liver Injury in Rats

HUANG Ying, TAN Shuming, CHEN Ping, CHEN Xiaomin, SONG Zhangjun   

  1. 1. School of Liquor and Food Engineering, Guizhou University, Guiyang 550025, China; 2. Guizhou Key Laboratory for Storage and Processing of Agricultural and Animal Products, Guiyang 550025, China
  • Published:2020-11-23

Abstract: Purpose: To investigate the protective effect of honey polyphenol extract (HPE) on acute alcoholic hepatic injury in rats. Methods: Rats were randomly divided into blank control, model control, drug control (100 mg/(kg mb·d)), low- and high-dose HPE groups (100 and 200 mg/(kg mb·d)). After intragastric administration for 30 consecutive days, all rats except those in the blank control group were administered with Chinese Baiju containing 56% (V/V) alcohol at a dose of 15 mL/kg mb by gavage to establish an acute liver injury model. Twelve hours later, serum transaminase activity and antioxidant and anti-inflammatory indexes in liver were measured. The relative mRNA expression levels of extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK) and p38 kinase (p38) were determined by real-time quantitative polymerase chain reaction (qPCR). Hematoxylin-eosin (HE) staining was performed to observe the pathological changes of liver tissues. Results: Compared with the model group, the levels of alanine aminotransferase (ALT), aspartate aminotransaminase (AST), malondialdehyde (MDA) and tumor necrosis factor alpha (TNF-α) were decreased in the HPE treatment groups, and the activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) and the level of reduced glutathione (GSH) were increased. Moreover, HPE downregulated the relative mRNA expression of ERK, JNK and p38, and improved the pathological changes of liver tissues. Conclusion: HPE has a protective effect on acute alcoholic liver injury in rats, and the underlying mechanism may be related to its antioxidant and anti-inflammatory activities.

Key words: honey polyphenol extract; acute alcoholic liver injury; protective effect; antioxidant activity; anti-inflammatory activity

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