FOOD SCIENCE ›› 2021, Vol. 42 ›› Issue (3): 158-164.doi: 10.7506/spkx1002-6630-20191229-340

• Nutrition & Hygiene • Previous Articles     Next Articles

Intervention Effect of Chitooligosaccharide on Alcohol-Induced Intestinal Damage in Rats

XU Ying, WANG Bin, JIANG Qixing, XIA Wenshui, XU Yanshun   

  1. (1. School of Food Science and Technology, Jiangnan University, Wuxi 214122, China; 2. Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, Wuxi 214122, China)
  • Published:2021-02-25

Abstract: Objective: To investigate the intervention effect of chitooligosaccharide (COS) on intestinal injury induced by chronic drinking. Methods: Male Wistar rats were randomly divided into five groups: blank control, model, low-, medium-, and high-dose COS treatment groups. The rats in all groups except the blank control group were intragastrically administered with 4.5 g/kg mb of alcohol for 42 successive days to induce intestinal injury, which was preceded by COS for the treatment groups. Results: COS could significantly improve the performance of the rat duodenal?smooth muscle (P < 0.05), restore small intestinal health, significantly reduce the concentration of D-lactic acid (D-LA), diamine oxidase (DAO) activity, the levels of pro-inflammatory cytokines including interleukin (IL)-1β, tumor necrosis factor (TNF)-α and IL-6, malondialdehyde (MDA) concentration and protein carbonyl content in plasma (P < 0.05) and claudin-4 gene expression (P < 0.05), and significantly inhibit the gene expression of claudin-4 (P < 0.05). High-dose COS could significantly increase the gene expression of the tight junction proteins occludin and ZO-1 (P < 0.05). Medium- and high-dose COS could significantly reduce gene the expression of IL-6, IL-1β and TNF-α in the duodenum (P < 0.05). Conclusion: COS alleviates intestinal damage caused by chronic drinking in rats by improving small intestinal health, enhancing intestinal mucosal barrier, reducing inflammatory responses and alleviating oxidative damage.

Key words: chitooligosaccharide; alcohol; intestinal mucosal barrier; inflammatory response; oxidative damage

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