Abstract: In this study, the effects of addition of bamboo leaf extract (BLE) and knockout of the transcriptional factor DAL80 gene on the regulation of arginine (Arg) metabolism were investigated. It was found that both DAL80 knockout and BLE addition affected arginine metabolism, but by different mechanisms. DAL80 knockout could improve the transcriptional level of urea metabolism-related genes (DUR1,2, DUR3 and DAL82), accelerating the consumption of ethyl carbamate precursors by Saccharomyces cerevisiae to inhibit the formation of ethyl carbamate. BLE reduced the utilization of Arg by S. cerevisiae and consequently the synthesis of urea, finally reducing the formation of ethyl carbamate. When working simultaneously, BLE could weaken the inhibitory effect of DAL80 knockout, but the effect of DAL80 knockout on nitrogen metabolism in S. cerevisiae was still dominant.

Key words: Chinese yellow rice wine; arginine; urea; transcriptional factor DAL80; bamboo leaf extract