Synthesis, Characterization and Antioxidant Activity of Millet Bran Soluble Dietary Fiber-Cr(III) Complexes

doi:10.7506/spkx1002-6630-20200902-023

Abstract

Abstract: Soluble dietary fiber (SDF) extracted from millet bran by enzymatic hydrolysis was coordinated with trivalent chromium ions in a weakly alkaline environment to produce SDF-Cr(III) complexes. Scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR) and atomic force microscopy (AFM) were used to characterize the structures of SDF and SDF-Cr(III) complexes. Gel permeation chromatography (GPC) and high-performance liquid chromatography (HPLC) were used to determine their molecular mass distribution and monosaccharide composition. Their in vitro antioxidant properties were measured by 1,1-diphenyl-2-trinitrophenylhydrazine (DPPH) radical, 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radicla cation, and hydroxyl radical scavenging capacity and total antioxidant capacity. The results showed that the surface structure of SDF-Cr(III) was loose and porous, exhibiting a decrease in the aggregation degree of island-like bulges and more scattered intermolecular arrangements compared to SDF. A large difference was found between SDF and SDF-Cr(III) in functional groups. According to FTIR analysis, SDF-Cr(III) showed reduced stretching vibrations of the active functional groups (hydroxyl, ester, and carbonyl groups), a blue shift in the absorption maximum, and a Cr-O stretching vibration at 530.27 cm-1, confirming the binding Cr3+ to SDF. The number average molecular mass (mn), weight average molecular mass (mw) and molecular mass distribution width index (D) of SDF was 0.195 × 104 Da, 0.940 × 104 Da, and 4.81, while those of SDF-Cr(III) were 0.742 × 104 Da, 4.708 × 104 Da and 6.35, respectively as determined by GPC. HPLC showed that the relative content of monosaccharides in SDF-Cr(III) was higher than that in SDF. SDF-Cr(III) had stronger in vitro antioxidant properties compared to SDF. In conclusion, millet bran SDF-Cr(III) may have the potential to be developed into health products with hypoglycemic effect.

Key words: millet bran; soluble dietary fiber; complex; structure; antioxidant activity

CLC Number:

TS218

QUAN Zhigang, WANG Weihao, ZHAO Shuting, LIU Dezhi, WANG Yifei, WU Yunjiao, SU Youtao, WEI Chunhong, CAO Longkui. Synthesis, Characterization and Antioxidant Activity of Millet Bran Soluble Dietary Fiber-Cr(III) Complexes[J]. FOOD SCIENCE, 2021, 42(21): 46-55.

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Synthesis, Characterization and Antioxidant Activity of Millet Bran Soluble Dietary Fiber-Cr(III) Complexes

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