Astaxanthin Protects Retinal Pigment Epithelial Cells from Oxidative Stress Induced by Blue Light Emitting Diodes

doi:10.7506/spkx1002-6630-20201017-154

FOOD SCIENCE ›› 2021, Vol. 42 ›› Issue (21): 128-136.doi: 10.7506/spkx1002-6630-20201017-154

• Nutrition & Hygiene • Previous Articles Next Articles

Astaxanthin Protects Retinal Pigment Epithelial Cells from Oxidative Stress Induced by Blue Light Emitting Diodes

LIU Ying, MA Ning, LIU Yaojie, GUO Yatu, LI Heyu, SHAN Yanqin, CHEN Yunxia, WANG Hao

(1. College of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin 300457, China; 2. Graduate School of Chengde Medical University, Chengde 067000, China; 3. Cangzhou People’s Hospital, Cangzhou 061000, China; 4.Tianjin Eye Hospital, Tianjin 300020, China; 5. Tianjin Ubasio Biotechnology Group Co., Ltd., Tianjin 300457, China; 6. Jiangsu Natural Food Co., Ltd., Taizhou 225700, China)

Online:2021-11-15 Published:2021-11-23

Abstract

Abstract: Objective: To investigate the protective effect of astaxanthin on oxidative stress injury induced by blue light-emitting diode (LED) in ARPE-19 cells and its underlying mechanism. Methods: The cells were pretreated with different concentrations of astaxanthin for 1 h followed by exposure to blue light LED for 24?h to induce photooxidative damage. The cell viability was determined by the MTT method, the cytotoxicity was determined by the lactate dehydrogenase (LDH) assay, the level of intracellular reactive oxygen species (ROS) was measured using 6-carboxy-2’,7’-dichorodihydrofluorescein diacetate (DCFH-DA) as a fluorescence probe, the change of intracellular mitochondrial membrane potential was measured using the fluorescence probe JC-1, and the activity of endogenous antioxidant enzymes was determined by commercial kits. The transcriptional expression level of phase II detoxification genes was measured by real-time fluorescence quantitative polymerase chain reaction (RT-qPCR), and the nuclear protein expression level of nuclear factor erythroid-related factor 2 (Nrf2) was measured by Western blot. Results: Astaxanthin (at 5, 10 and 20?μmol/L) pretreatment inhibited the decrease in the cell viability induced by blue light LED in a concentration-dependent manner, alleviated the cytotoxicity, reduced the production of intracellular ROS, stabilized the mitochondrial membrane potential and increased the activity of endogenous antioxidant enzymes. In addition, astaxanthin induced nuclear translocation of Nrf2 and increased the expression of antioxidant enzymes and phase II detoxification genes, thus protecting ARPE-19 cells from oxidative stress induced by blue light LED. Conclusion: Astaxanthin protects ARPE-19 cells from oxidative stress induced by blue light LED by inducing nuclear translocation of Nrf2 and promoting the expression of antioxidant enzymes and phase II detoxification genes.

Key words: astaxanthin; ARPE-19 cells; blue light light-emitting diodes; oxidative stress; nuclear factor erythroid-2-related factor 2

CLC Number:

TS201.4

LIU Ying, MA Ning, LIU Yaojie, GUO Yatu, LI Heyu, SHAN Yanqin, CHEN Yunxia, WANG Hao. Astaxanthin Protects Retinal Pigment Epithelial Cells from Oxidative Stress Induced by Blue Light Emitting Diodes[J]. FOOD SCIENCE, 2021, 42(21): 128-136.

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Astaxanthin Protects Retinal Pigment Epithelial Cells from Oxidative Stress Induced by Blue Light Emitting Diodes

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