Development and Application of Broad-Spectrum Aptamer-Magnetic Separation Assisted Fluorescence Method for Determination of Organophosphorus Pesticide Residues in ‘Shatangju’ Mandarine

doi:10.7506/spkx1002-6630-20201114-156

FOOD SCIENCE ›› 2022, Vol. 43 ›› Issue (4): 264-269.doi: 10.7506/spkx1002-6630-20201114-156

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Development and Application of Broad-Spectrum Aptamer-Magnetic Separation Assisted Fluorescence Method for Determination of Organophosphorus Pesticide Residues in ‘Shatangju’ Mandarine

HU Chaoqiong, WANG Lijun, TAO Xuan, ZHU Yun, LIANG Yu, YANG Xiao, CHEN Xianggui, HUANG Yukun

(1. School of Food and Biological Engineering, Xihua University, Chengdu 610039, China; 2. Key Laboratory of Food Non Thermal Processing, Engineering Technology Research Center of Food Non Thermal Processing, Yibin Xihua University Research Institute, Yibin 644004, China; 3. Chengdu Brilliant Pharmaceutical Co. Ltd., Chengdu 610041, China)

Online:2022-02-25 Published:2022-03-08

Abstract

Abstract: In this paper, a magnetic separation assisted fluorescence method for the determination of three common organophosphorus pesticides (OPPs) in ‘Shatangju’ mandarin was established. Magnetic beads were synthesized by solvothermal method and modified by polypyrrole (PPy) to form Fe3O4@PPy nanoparticles as a fluorescence quenching agent. This method was based on the use of a 6-carboxyfluorescein (FAM)-labeled aptamer (S4-29) by virtue of its broad-spectrum recognition ability for OPPs. When Fe3O4/PPy nanoparticles mass ratio, Fe3O4@PPy nanoparticles concentration and incubation time were 1:1.5, 0.2 mg/mL, and 120 min, respectivley, the detection limits of phorate, omethoate and profenofos were 4.17 × 10-4, 2.77 × 10-4, and 4.67 × 10-4 μg/kg, and the recoveries ranged from 79.94% to 108.00% with relative standard deviations (RSDs) between 3.05% and 7.39%. In conclusion, this method has strong selectivity and high accuracy and can be used for the analysis of banned OPPs in agricultural products.

Key words: aptamer; nanomagnetic beads; organophosphorus pesticides; fluorescence analysis; ‘Shatangju’ mandarin

CLC Number:

TS207.3

HU Chaoqiong, WANG Lijun, TAO Xuan, ZHU Yun, LIANG Yu, YANG Xiao, CHEN Xianggui, HUANG Yukun. Development and Application of Broad-Spectrum Aptamer-Magnetic Separation Assisted Fluorescence Method for Determination of Organophosphorus Pesticide Residues in ‘Shatangju’ Mandarine[J]. FOOD SCIENCE, 2022, 43(4): 264-269.

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Development and Application of Broad-Spectrum Aptamer-Magnetic Separation Assisted Fluorescence Method for Determination of Organophosphorus Pesticide Residues in ‘Shatangju’ Mandarine

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