Effect of the Inhibition of PDIDSM_85260 and PDIDSM_08010 Expression by RNA Interference on the Biotransformation of Limonene to α-Terpineol

doi:10.7506/spkx1002-6630-20210115-171

FOOD SCIENCE ›› 2022, Vol. 43 ›› Issue (6): 141-148.doi: 10.7506/spkx1002-6630-20210115-171

• Bioengineering • Previous Articles Next Articles

Effect of the Inhibition of PDIDSM_85260 and PDIDSM_08010 Expression by RNA Interference on the Biotransformation of Limonene to α-Terpineol

ZHANG Lulu, FAN Gang, LI Xiao, REN Jingnan, PAN Siyi, HUANG Wen, HE Jin

(1. College of Food Science and Technology, Henan University of Technology, Zhengzhou 450001, China; 2. Key Laboratory of Environment Correlative Dietology, Ministry of Education,College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China; 3. College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China)

Online:2022-03-25 Published:2022-03-28

Abstract

Abstract: Our previous results have shown that some genes such as PDIDSM_85260 and PDIDSM_08010 may be partly responsible for limonene biotransformation to α-terpineol as determined by analysis of genomic and transcriptomic data, protein separation and purification, and mass spectrometry. In order to explore the biological functions of the genes, RNA interference (RNAi) vectors for PDIDSM_85260 and PDIDSM_08010 were constructed and transformed into Penicillium digitatum DSM62840 by Agrobacterium tumefaciens-mediated transformation. RANi strains were obtained by a series of experiments, and the functions of the two genes were analyzed in P. digitatum DSM62840. It was found that the amount of α-terpineol produced by the silenced transformants obviously decreased compared to the wild-type strain, suggesting that PDIDSM_85260 and PDIDSM_08010 may participate in limonene biotransformation to α-terpineol.

Key words: limonene biotransformation; α-terpineol; RNAi; PDIDSM_85260; PDIDSM_08010

CLC Number:

ZHANG Lulu, FAN Gang, LI Xiao, REN Jingnan, PAN Siyi, HUANG Wen, HE Jin. Effect of the Inhibition of PDIDSM_85260 and PDIDSM_08010 Expression by RNA Interference on the Biotransformation of Limonene to α-Terpineol[J]. FOOD SCIENCE, 2022, 43(6): 141-148.

[1]	Yu-Tong LIN. Clonal expression of artificially designed peptides PF1 and PF2 and their effect on glucose oxidase activity [J]. FOOD SCIENCE, 0, (): 0-0.
[2]	. Effects of Inhibition of PDIDSM_85260 and PDIDSM_08010 by RNAi on the Biotransformation of Limonene to α-Terpineol [J]. , 0, (): 0-0.
[3]	Cheng Peng NI Hui. Cloning, expression, characterization and clarification of α-L-arabinofuranoside from Aspergillus niger [J]. , 0, (): 0-0.
[4]	PENG Cheng, XIAO Wenxi, NI Hui, LI Lijun, TAN Wansen, LIN Yanling, LI Qingbiao. Cloning, Expression and Characterization of α-L-Arabinofuranosidase from Aspergillus niger and Its Clarification Effect on Fruit Juice [J]. FOOD SCIENCE, 2022, 43(2): 83-92.
[5]	Yong-Si LIANG. Construction of Corynebacterium glutamicum capable of biosynthesis of glyoxylic acid using CRISPRi [J]. , 0, (): 0-0.
[6]	LIANG Yongsi, SHEN Kaijia, FAN Xuyun, HAN Wuyang, LI Tianming. Engineering of Corynebacterium glutamicum for Biosynthesis of Glyoxylic Acid Using Clustered Regularly Interspaced Short Palindromic Repeats Interference [J]. FOOD SCIENCE, 2021, 42(2): 170-176.
[7]	LI Ling, GONG Jinyan, YUAN Haina, FANG Ruosi, CHU Bingquan, XIAO Gongnian, Namsoo HAN. Characterization of D-Lactate Dehydrogenase from Leuconostoc mesenteroides ATCC8293 [J]. FOOD SCIENCE, 2020, 41(18): 153-158.
[8]	CHEN Wei, GU Xinxi, HUANG Lei, LI Chen, TIAN Hongtao, LU Haiqiang. Expression in Pichia pastoris and Characterization of a Thermophilic Mannanase and Its Application in Juice Clarification [J]. FOOD SCIENCE, 2019, 40(22): 81-87.
[9]	Kiwi Chow. Effects of Mechanical Force of Turning over to the Formation of Aliphatic Aroma [J]. FOOD SCIENCE, 0, (): 0-0.
[10]	JIANG Dongjian, LUO Xiu’er, HE Qinghua. Phage Displayed Nanobody Mediated Green Immuno-PCR for Detection of Deoxynivalenol [J]. FOOD SCIENCE, 2019, 40(8): 256-261.
[11]	嘉欧阳. Biosynthesis of Trehalose from Cellobiose by Recombinant Escherichia coli [J]. FOOD SCIENCE, 0, (): 0-0.
[12]	SHI Yunfeng, ZHANG Tong, CHEN Qin. Prokaryotic Expression of Peanut Allergen Ara h 1 and Its Allergenicity [J]. FOOD SCIENCE, 2019, 40(6): 121-127.
[13]	NIU Xiaodi, LI Tianming, LIU Jinlei, YANG Tianyong, LI Ziyi, FENG Huiyong. Gene Deletion in Saccharomyces cerevisiae Using CRISPR-Cas9 System [J]. FOOD SCIENCE, 2019, 40(6): 151-158.
[14]	LI Xin, ZHENG Zhaojuan, YUE Taiwen, OUYANG Jia. Biosynthesis of Trehalose from Cellobiose by Recombinant Escherichia coli [J]. FOOD SCIENCE, 2019, 40(6): 180-186.
[15]	Yunfeng Shi qin chen. Prokaryotic expression of peanut allergen Ara h 1 and its allergenicity [J]. FOOD SCIENCE, 0, (): 0-0.

Effect of the Inhibition of PDIDSM_85260 and PDIDSM_08010 Expression by RNA Interference on the Biotransformation of Limonene to α-Terpineol

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments