Study on the profile of Chinese human milk oligosaccharides

Abstract

Abstract: Aim: To analyze the impact of different regions, lactation time, and blood type genotypes on HMOs content in healthy lactating mothers’ breast milk in representative regions of China, and to explore the composition characteristics of milk oligosaccharides in China. Methods: Using the method of longitudinal cohort study, this research collected breast milk samples from 109 healthy mothers at three lactation stages in 6 cities in east, west, north and south of China. The contents of 23 kinds of HMOs were analyzed by ultra-high performance liquid chromatography tandem triple quadrupole mass spectrometry. Results: The contents of total HMOs and total Fucosyl neutral oligosaccharides in Kunming, Jinan and Shenzhen were significantly higher than those in Beijing, Baotou and Xining, and the contents of 3-FL, LDFT and LNnDFH-II in Baotou were significantly lower than those in Shenzhen and Jinan, Beijing, respectively; Eight neutral oligosaccharides (LNT, LNnT, LNFP-I,LNDFH-II, LNDFH-I&LNnDFH-I, MFLNH-I, DFpLNnH and DFLNHa) and four acidic oligosaccharides (6’-SL, DSLNT, 3’-SLNFP-II and 6’-SLNFP-VI) showed a significant decreasing trend with lactation time; 3-FL increased significantly with lactation time, LDFT and LNnDFH-II also increased with lactation time, but the difference was not significant; The contents of two kinds of mon-ofucosylhexasaccharides (MFLNnH and MFLNH-III) reached the highest in 1-month-old mature milk, and then remained unchanged or decreased slightly; According to the genotyping of blood groups inferred from oligosaccharide types, it was found that the lactating mothers in all cities were mainly Se+Le+, but the contents of other 18 oligosaccharides in this type of breast milk were lower than those of other blood groups except that the contents of LNDFH-II, LNDFH-I & LNnDFH-I, 6’-SL and LSTc were higher than those of other blood groups. Conclusion: This study revealed that region, lactation stage and maternal blood genotype lead to differences in the profile of human milk oligosaccharides, which provides scientific theoretical guidance for the accuracy of breast milk oligosaccharides in the nutritional intake of infants and young children in the early life stage.

Key words: human milk oligosaccharides, region, lactation, blood genotype

CLC Number:

Li-juan MI. Study on the profile of Chinese human milk oligosaccharides[J]. FOOD SCIENCE, 0, (): 0-0.

References

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