Abstract: In order to explore the microfloral succession in Takifugu flavidus during storage at 0 ℃, the spoilage bacteria were detected by 16S rDNA sequencing, the dominant spoilage bacteria in the late stage of storage were identified, and the changes in the microbial flora and the spoilage capacity of the dominant spoilage bacteria were analyzed. The results showed that the refrigerated shelf life of T. flavidus was about 5 days. In the early stage of storage (0–3 days), T. flavidus had the highest number of species of bacteria, including Pseudomonas, Streptophyta, and Shewanella, while in the late stage (6–15 days), the bacterial species were concentrated mainly in Pseudomonas and Shewanella. Ten dominant spoilage bacteria were isolated and identified, Shewanella and Pseudomonas accounting for a high proportion. Moreover, the spoilage capacity of several common dominant spoilage bacteria was analyzed, revealing that the decreasing order of the spoilage capacity was Pseudomonas fluorescent > P. azotoformans > Shewanella hafniensis > S. baltica. The results of this study provide a reference for understanding the spoilage mechanism of T. flavidus during cold storage and extending its shelf life.

Key words: Takifugu flavidus; high-throughput sequencing; bacterial community succession; dominant spoilage bacteria; spoilage capacity