Abstract: Abstract: Protein-polysaccharide complexes of Agaricus bisporus, Stropharia rugosa and Morchella esculenta were prepared. The contents of protein, polysaccharide, amino acids and monosaccharides were analyzed, establishment of hyperlipemia model in vitro by oleic acid-induced liver cell line LO-2, and establishment of hyperlipemia model in mice based on high-fat diet, the lipid-lowering effects of protein-polysaccharide complexes of Agaricus bisporus, Stropharia rugosa and Morchella esculenta were investigated in vitro and in vivo. The results showed that the protein content of protein-polysaccharide complex was 55.56±0.51% , 52.41±0.31% and 54.96±0.47% , and the content of polysaccharide was 21.98±1.17% , 31.95±0.52% and 15.55±0.78% , respectively, for Agaricus bisporus, Stropharia rugosa and Morchella esculenta. The results of cell experiments in vitro showed that the protein-polysaccharide complexes of three edible fungi could significantly inhibit the accumulation of lipid droplets in hepatocytes, protein-polysaccharide complex of Agaricus bisporus, Stropharia rugosa and Morchella esculenta can significantly inhibit the increase of body weight, TG and LDL-C levels, and block the swelling of adipose tissue cells in mice fed high-fat diet, inhibit lipid deposition in liver tissue. In the high-dose group, the contents of lipid in the livers of the mice with hyperlipidemia were reduced from 30.59±0.38% to 13.74±1.57% , 9.68±0.73% and 7.15±0.39% , respectively, by protein-polysaccharide complex of Agaricus bisporus, Stropharia rugosa and Morchella esculenta, the inhibition rate of hepatic lipid droplets was 55.09±5.12% , 68.34±2.37% and 76.64±1.28% , respectively. In addition, Agaricus bisporus, Stropharia rugosa and Morchella esculenta protein-polysaccharide complex had good effects on the liver function damage caused by long-term high-fat diet. In summary, studies have shown that Agaricus bisporus, Stropharia rugosa and Morchella esculenta protein-polysaccharide complexes have the effects of regulating lipid metabolism, alleviating liver injury, and alleviating hyperlipidemia, for its related function product development provides the reference.

Key words: Keywords: edible fungi, protein, polysaccharide, hypolipidemic