FOOD SCIENCE ›› 2025, Vol. 46 ›› Issue (4): 147-153.doi: 10.7506/spkx1002-6630-20240704-045

• Component Analysis • Previous Articles    

Comparative Metabolomic Analysis of Shrimp Pastes from Different Regions

LIU Jiale, ZHENG Lanping, LIU Ying, LIU Min, CHEN Yaquan, LONG Zhao, FU Xiangjin   

  1. (1. College of Food Science and Engineering, Central South University of Forestry & Technology, Changsha 410004, China; 2. Changsha Environmental Protection College, Changsha 410004, China; 3. Hunan Province Seasonings Green Manufacturing Engineering Technology Research Center, Hunan Province AffBio Technology Co. Ltd., Liuyang 410323, China; 4. Hunan Xiangdian Food Co. Ltd., Liuyang 410300, China)
  • Published:2025-02-07

Abstract: Objective: To study the composition of non-volatile small molecules in shrimp pastes from different geographical origins. Methods: Metabolomics based on ultra-high performance liquid chromatography-Q Exactive high field-mass spectrometry was used to detect metabolites in shrimp pastes from Shandong, Guangdong and Hebei. The characteristic metabolites of shrimp paste from each region were identified by orthogonal partial least square-discriminant analysis (OPLS-DA). Results: Shrimp paste was rich in amino acid and fatty acid metabolites, forming a large number of N-acyl compounds, with cinnamic acid, 11’-carboxy-α-chromanol and β-leucine being the major ones. It was found that shrimp paste also contained active neurotransmitters such as dopamine, norepinephrine, 6-hydroxymelatonin, and γ-glutamine-5-hydroxytryptophan. The major characteristic metabolites of Shandong shrimp paste were cinnamic acid and β-leucine. 12-Hete-T and N-oleyl taurine were the major characteristic metabolites in Guangdong shrimp paste. The major metabolites of Hebei shrimp paste were eicosapntemacnioc acid (EPA) and cyanidin-3-O-(6’-malonyl-arabinoside). Conclusion: Shrimp paste is rich in small molecules such as β-leucine, glutamic acid, proline-valine and leucine-glycine as well as bioactive ingredients such as taurine, norepinephrine and dopamine. The composition of metabolites in shrimp paste is region specific.

Key words: shrimp paste; metabolomics; ultra-high performance liquid chromatography-Q Exactive high field-mass spectrometry; orthogonal partial least square-discriminant analysis; characteristic metabolites

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