Protective effect of Ganoderma atrum polysaccharides against cadmium-induced renal cell injury in human embryos

Abstract

Abstract: Objective: To investigate the protective effect of Ganoderma atrum polysaccharides (GAP) on Cadmium (Cd)-induced injury in human embryonic kidney cells (HEK-293). Methods: A cell model was established with 2 μg/mL Cd exposure, and 100 μg/mL GAP was used for intervention. The cell viability of different treatment groups of HEK-293 was determined using the CCK-8 assay. The activities of lactate dehydrogenase (LDH), catalase (CAT), and superoxide dismutase (SOD) were measured, as well as the levels of glutathione (GSH) and malondialdehyde (MDA). The levels of reactive oxygen species (ROS) and mitochondrial membrane potential were determined using a fluorescence plate reader. The expression of antioxidant-related genes (HO-1, Keap1, Nfr2) and mitochondrial autophagy-related genes (PINK1, Parkin, P62, LC3) was measured by Q-PCR. Results: 100 μg/mL GAP could inhibit Cd-induced cytotoxicity and significantly increase cell viability (P < 0.05); it decreased LDH, ROS, and MDA levels, and increased GSH, CAT, and SOD activities as well as mitochondrial membrane potential levels (P < 0.05), and upregulated the expression of Nrf2 and HO-1, while downregulating the expression of Keap1, PINK1, Parkin, P62, and LC3 (P < 0.05). Conclusion: GAP has a protective effect on Cd-induced injury in human embryonic kidney cells by alleviating Cd-induced oxidative stress and excessive mitochondrial autophagy.

Key words: Keywords: Ganoderma atrum polysaccharides(GAP), Cadmium(Cd), antioxidant, mitochondrial autophagy

CLC Number:

R151.2

linchun wang Qing-Hai ZHANG. Protective effect of Ganoderma atrum polysaccharides against cadmium-induced renal cell injury in human embryos[J]. FOOD SCIENCE.

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