Evaluation of the Anti-allergic Activity of Bovine Milk Exosomes Using Mast Cell Degranulation Model

doi:10.7506/spkx1002-6630-20241104-016

FOOD SCIENCE ›› 2025, Vol. 46 ›› Issue (12): 226-232.doi: 10.7506/spkx1002-6630-20241104-016

• Nutrition & Hygiene • Previous Articles Next Articles

Evaluation of the Anti-allergic Activity of Bovine Milk Exosomes Using Mast Cell Degranulation Model

FU Siqi, LIU Yan, ZHANG Chao, YANG Fan, XIE Ruofan, WANG Xiaodong, LI Xin, CHEN Hongbing

(1. State Key Laboratory of Food Science and Resources, Nanchang University, Nanchang 330047, China;2. College of Food Science & Technology, Nanchang University, Nanchang 330047, China;3. Jiangxi Provincial Key Laboratory of Food Allergy, Nanchang University, Nanchang 330047, China;4. Sino-German Joint Research Institute, Nanchang University, Nanchang 330047, China)

Online:2025-06-25 Published:2025-05-23

Abstract

Abstract: A degranulation model was established using mouse bone marrow-derived mast cell (BMMC) to investigate the anti-allergic activity of milk exosomes (M-Exo). Casein was removed from cow’s milk using a rennet-assisted method, and then M-Exo was isolated from the milk by ultracentrifugation and characterized. Different concentrations of M-Exo were used to treat sensitized BMMCs, and the release rate of activated β-hexosaminidase (β-HEX) and cytokine secretion were measured. The results showed that the prepared M-Exo had a typical tea tray-like bilayer membrane structure, with particle size ranging from 30 to 200 nm. M-Exo contained exosome marker proteins CD81, tumor susceptibility gene 101 (TSG101) and heatshockprotein 70 (HSP70). It was further found that M-Exo could be taken up by BMMCs. In the degranulation model, it was confirmed that M-Exo could significantly inhibit the release rate of β-HEX and downregulate the expression of tumor necrosis factor-α (TNF-α), interleukin-4 (IL-4), and IL-13 in a dose-dependent manner, indicating that M-Exo has anti-allergic activity.

Key words: milk exosomes; characterization; mast cells; degranulation; anti-allergic

CLC Number:

TS201.4

FU Siqi, LIU Yan, ZHANG Chao, YANG Fan, XIE Ruofan, WANG Xiaodong, LI Xin, CHEN Hongbing. Evaluation of the Anti-allergic Activity of Bovine Milk Exosomes Using Mast Cell Degranulation Model[J]. FOOD SCIENCE, 2025, 46(12): 226-232 https://doi.org/10.7506/spkx1002-6630-20241104-016

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Evaluation of the Anti-allergic Activity of Bovine Milk Exosomes Using Mast Cell Degranulation Model

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