Fucoxanthin-Loaded Oil-in-Water (O/W) Emulsion Stabilized by Whey Protein Isolate-Docosahexaenoic Acid-Containing Phospholipid Complexes: Characterization, Biocompatibility and Effect on RAW264.7 Cells

doi:10.7506/spkx1002-6630-20250709-076

Abstract

Abstract: This study developed an oil-in-water (O/W) emulsion using complexes of whey protein isolate (WPI) and docosahexaenoic acid-containing phospholipid (DHAPL) as emulsifiers for encapsulating fucoxanthin (FX), aiming to enhance its thermal stability, antioxidant capacity, bioaccessibility, and protective effects against oxidative damage in RAW264.7 macrophages. The emulsions stabilized by WPI-DHAPL complexes demonstrated excellent encapsulation efficiency for FX (> 94%), significantly surpassing the WPI-stabilized emulsion, with long-chain DHAPL (e.g., DHA-containing phosphatidyl monopalmitoyl glycerol (DHAPL-MP) and DHA-containing phosphatidyl monostearate glycerol (DHAPL-MG)) being more effective in this regard. The structure of the mixed emulsifiers endowed the emulsion with outstanding thermal stability, maintaining over 80% of FX under harsh conditions (75 ℃/0.5 h), and the FX-loaded emulsion exhibited excellent storage stability at 25 ℃. Furthermore, the DHAPL-WPI system overcame the key limitation on the bioavailability of hydrophobic bioactive substances, resulting in 70% FX bioaccessibility during in vitro simulated digestion, which was 2.8 times higher than that of free FX. The dual-interfacial antioxidant mechanism of the WPI-DHAPL complex significantly enhanced the free radical scavenging capacity of FX, increasing the 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) cation racial and 1,1-diphenyl-2-picrylhydrazyl (DPPH) racial scavenging rates by 35%–42%. It also provided strong protection against oxidative stress in RAW264.7 macrophages, with medium- and short-chain DHAPL showing more pronounced effects. The bioactive substance delivery system developed in this study provides an effective encapsulation solution for functional food development, successfully overcoming the dual bottlenecks of thermal instability and low intestinal absorption efficiency of hydrophobic active ingredients in the food industry.

Key words: fucoxanthin; whey protein isolate; phospholipid; oil-in-water emulsion; stability; bioaccessibility

CLC Number:

TS201.1

HE Chenxi, CHEN Xi, DIAO Rujing, ZHANG Xinyuan, SUN Jian’an. Fucoxanthin-Loaded Oil-in-Water (O/W) Emulsion Stabilized by Whey Protein Isolate-Docosahexaenoic Acid-Containing Phospholipid Complexes: Characterization, Biocompatibility and Effect on RAW264.7 Cells[J]. FOOD SCIENCE, 2026, 47(2): 66-75.

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Fucoxanthin-Loaded Oil-in-Water (O/W) Emulsion Stabilized by Whey Protein Isolate-Docosahexaenoic Acid-Containing Phospholipid Complexes: Characterization, Biocompatibility and Effect on RAW264.7 Cells

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