Abstract: This study used Fe-based meta-organic frameworks (MOFs) as the carrier and laccase (Lac) as the functional component to synthesize Lac@Fe-1,3,5-benzenetricarboxylic acid (BTC) biocomposite through an in situ encapsulation method. The removal efficiency and mechanism of this composite for aflatoxin B1 (AFB1) and zearalenone (ZEN) in edible oil were systematically investigated. Results indicated that the thermal stability of laccase improved after immobilization on MOFs, and the removal rates of AFB1 and ZEN by the immobilized laccase were 71.64% and 60.98% in peanut oil, respectively. After four reuse cycles, the removal rates remained at 52.47% and 46.82%, respectively, significantly higher than those of free laccase. Furthermore, structural identification and zebrafish toxicity experiments were conducted on mycotoxins and their degradation products, confirming a significant reduction in their toxicity. Compared with free laccase, the immobilized enzyme demonstrated superior efficiency, stability, and reusability for mycotoxin removal. Therefore, this approach exhibits promising potential for mycotoxin decontamination applications.

Key words: immobilized laccase; metal-organic frameworks; peanut oil; mycotoxins degradation; safety