Study on Alkaline Protease from Bacillus licheniformis A

doi:10.7506/spkx1002-6630-201101047

FOOD SCIENCE ›› 2011, Vol. 32 ›› Issue (1 ): 199-202.doi: 10.7506/spkx1002-6630-201101047

• Bioengineering • Previous Articles Next Articles

Study on Alkaline Protease from Bacillus licheniformis A

ZHANG Wen-jie，GAO Xiang，SUN Jing，JIANG Li-yan，DING Jin-feng，YUE Fu-peng，SU Xiu-rong*

Faculty of Life Science and Biotechnology, Ningbo University, Ningbo 315211, China

Received:2010-09-30 Revised:2010-12-20 Online:2011-01-15 Published:2010-12-28
Contact: SU Xiu-rong E-mail:suxiurong@nbu.edu.cn

Abstract

Abstract:

The alkaline protease produced by the fermentation of Bacillus licheniformis A, a strain isolated from the hydrolysate of 60Co-irradiated Acaudina leucoprocta was salted out from the fermentation supernatant with ammonium sulfate. After DEAESepharose ion exchange chromatographic separation and subsequent Sephadex G-75 gel permeation chromatographic purification, an alkaline protease yield of 5.8% was achieved. The alkaline protease was determined by SDS-PAGE to have a molecular weight of 35 kD. The optimal reaction temperature and pH of the enzyme were 50 ℃ and 11, respectively. The enzyme had perfect thermostability and a certain level of resistance to oxidation.

Key words: Bacillus licheniformis A, alkaline protease, isolation and purification

CLC Number:

Q814.1

ZHANG Wen-jie，GAO Xiang，SUN Jing，JIANG Li-yan，DING Jin-feng，YUE Fu-peng，SU Xiu-rong*. Study on Alkaline Protease from Bacillus licheniformis A[J]. FOOD SCIENCE, 2011, 32(1 ): 199-202.

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Study on Alkaline Protease from Bacillus licheniformis A

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