Isolation, Identification and Biological Activity of ECSYW-28 Strain

doi:10.7506/spkx1002-6630-201023045

FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (23): 198-203.doi: 10.7506/spkx1002-6630-201023045

• Bioengineering • Previous Articles Next Articles

Isolation, Identification and Biological Activity of ECSYW-28 Strain

ZHANG Xiao-ling1，YANG Qiao1,*，WU Wen-hui2

1. East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai 200090, China；
2. College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China

Received:2010-04-08 Revised:2010-11-13 Online:2010-12-15 Published:2010-12-29
Contact: YANG Qiao E-mail:cctcc2004@whu.edu.cn

Abstract

Abstract:

A new carotenoid-producing symbiotic bacterium was isolated from a poison-producing paralytic shellfish Gymnodium sp., and then the heterogeneous taxonomic identification of this strain was performed. The 16S rRNA sequence (GU385809) homology analysis exhibited that the strain had 95.1% similarity with Kocuria rosea DSM 20447T. The cell wall was mainly composed of fatty acids that were identified as anteiso C15:0 and iso C15:0 by Sherlock MIDI system. This strain was identified as a new strain Kocuria sp. ECSYW-28 belonging to the genus of Kocuria based on physiological and biochemical characteristics, BIOLOG identification system and molecular evolution analysis. Meanwhile, this strain was confirmed to have stronger resistance to 60Co γ-ray irradiation and H2O2 treatment. Liquid chromatography-mass spectrometry analysis showed that this strain could produce carotenoids that are composed ofβ -carotene and an unknown-structural carotenoid-compound B. Antioxidant capacity analysis was analyzed by using ESR spectroscopy to reveal stronger scavenging effect of compound B on superoxide anion free radicals, one of the major free radicals generated duringγ-ray ionizing irradiation. The phytoene synthase (crtB) and phytoene dehydrogenase (crtI) genes involved in carotenoid biosynthesis pathway in ECSYW-28 strain were subjected to PCR amplification and bioinformatic comparison to confirm the molecular basis of carotenoid biosynthesis in the strain.

Key words: poison-producing paralytic shellfish, resistance to irradiation, carotenoids, heterogeneous taxonomic identification, biosynthesis gene

CLC Number:

Q949.3

ZHANG Xiao-ling1，YANG Qiao1,*，WU Wen-hui2. Isolation, Identification and Biological Activity of ECSYW-28 Strain[J]. FOOD SCIENCE, 2010, 31(23): 198-203.

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Isolation, Identification and Biological Activity of ECSYW-28 Strain

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