FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (21): 69-71.doi: 10.7506/spkx1002-6630-201021015

• Basic Research • Previous Articles     Next Articles

Using TLC-bioautography to Screen Active Compounds in Rape Bee Pollen

SUN Li-ping1,2,DU Xia1,XU Xiang1,2,DONG Jie1,2,AN Zhong-yao1,YANG Jia-lin1   

  1. 1. Bee Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100093, China;
    2. Bee Product Center, National R&D Center for Agro-Food Processing, Beijing 100093, China
  • Received:2010-08-02 Online:2010-11-15 Published:2010-12-29
  • Contact: SUN Li-ping E-mail:caasun@126.com

Abstract:

TLC-bioautography was used to screen the DPPH radical scavengers and the antibacterial ingredients in rape bee pollen. The experimental results indicated the flavonoids as DPPH radical scavengers mainly existed in form of glycosides in rape bee pollen, and the acidicly hydrolyzed ethanol extract from rape bee pollen contained a variety of antioxidant compounds including quercetin and kaempferol. There were two small molecules with Rf values of 0.29 and 0.86 found to have antibacterial activity in the water extract from rape bee pollen. According to the results of color development reaction, the types of antibacterial components were polyphenols, tannin, flavonoids or saponins, etc.

Key words: rape bee pollen, TLC-bioautography, antioxidant activity, antibacterial activity

CLC Number: