Isolation, Purification and Monosaccharide Composition Analysis of Polysaccharide from Diaphragma of Juglans regia L.

doi:10.7506/spkx1002-6630-201021041

FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (21): 182-185.doi: 10.7506/spkx1002-6630-201021041

• Basic Research • Previous Articles Next Articles

Isolation, Purification and Monosaccharide Composition Analysis of Polysaccharide from Diaphragma of Juglans regia L.

GAO Li1，WANG Qiang2，PATIGUL Mahmut1

1. College of Life Science and Technology, Xinjiang University, Urumqi 830046, China；
2. Test Center of Physical and Chemical Characteristic, Xinjiang University, Urumqi 830046, China

Received:2010-02-08 Online:2010-11-15 Published:2010-12-29
Contact: PATIGUL Mahmut E-mail:patigulimahemuti@126.com

Abstract

Abstract:

The purpose of this work was to isolate and purify polysaccharide from diaphragma of Juglans regia L (PDJL) and analyze its monosaccharide composition. A water-soluble crude polysaccharide was obtained by water extraction, ethanol precipitation and freeze-drying. The crude polysaccharide was purified to obtain a fraction of PDJL-A11 on DEAE-cellulose52 column and SephadexG-100 column. The purity of PDJL-A11 was analyzed by UV spectrophotometer. The monosaccharide composition and molecular ratio of PDJL-A11 were analyzed by gas chromatography. The monosaccharide composition of PDJLA11 was composed of arabinose, fructose, mannose, rhamnose and glucose with a molar ratio of 2.11: 6.52: 8.81: 12.59: 15.58.

Key words: diaphragma of Juglans regia L., polysaccharide, purification, gas chromatography, monosaccharide composition

CLC Number:

TS201.23

GAO Li1，WANG Qiang2，PATIGUL Mahmut1. Isolation, Purification and Monosaccharide Composition Analysis of Polysaccharide from Diaphragma of Juglans regia L.[J]. FOOD SCIENCE, 2010, 31(21): 182-185.

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Isolation, Purification and Monosaccharide Composition Analysis of Polysaccharide from Diaphragma of Juglans regia L.

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