Determination of Monobutyrin by GC-MS

doi:10.7506/spkx1002-6630-201020084

FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (20): 402-405.doi: 10.7506/spkx1002-6630-201020084

• Analysis & Detection • Previous Articles Next Articles

Determination of Monobutyrin by GC-MS

YI Lu-yao1,2，ZHENG Wei-wan1,*，LIAO He-jing1

1. State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China；
2. Jiangxi Provicial Institute for Drug and Food Control, Nanchang 330046, China

Received:2010-05-27 Revised:2010-09-19 Online:2010-10-25 Published:2010-12-29
Contact: ZHENG Wei-wan1 E-mail:zheng3008@126.com

Abstract

Abstract:

A GC-MS method for quantitative analysis of monobutyrin was established. The determination parameters were the capillary column DB-5MS (30 × 0.25 mmm, 0.25μm), injection temperature of 280 ℃ programmed temperature increase from 100 to 150 ℃ with an increase rate of 10 ℃/min, then programmed temperature increase from 150 ℃ to final temperature of 250 ℃ at an increase rate of 30 ℃/min, helium flow rate of 1.0 mL/min and split ratio of 10:1, respectively. Results indicated that a good linear relationship between integral value (Y) of peak area and the content of monobutyrin (X) in the range of 0.5－10μg/mL was observed and the linear equation was Y=146961X－30250 (r=0.9997). Recovery rate of monobutyrin were 99.9% with the RSD of 1.7%. The limit of determination was 0.2 ng. This method is simple, accurate and reproducible so that it can be used for the determination of monobutyrin.

Key words: GC-MS, monobutyrin, determination

CLC Number:

TS207.3

YI Lu-yao1,2，ZHENG Wei-wan1,*，LIAO He-jing1. Determination of Monobutyrin by GC-MS[J]. FOOD SCIENCE, 2010, 31(20): 402-405.

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Determination of Monobutyrin by GC-MS

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