Cloning and Prokaryotic Expression of Squalene Synthase Gene from Schizochytrium limacinum

doi:10.7506/spkx1002-6630-201019057

FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (19): 263-267.doi: 10.7506/spkx1002-6630-201019057

• Bioengineering • Previous Articles Next Articles

Cloning and Prokaryotic Expression of Squalene Synthase Gene from Schizochytrium limacinum

ZHU Lu-ying1,2，ZHU Qing-hua3,2，ZHANG Xue-cheng2,*

1. School of Life Sciences, Ludong University, Yantai 264025, China；2. College of Marine Life Sciences, Ocean University of
China,Qingdao 266003, China ；3. Department of Biology, Dezhou College, Dezhou 253023, China

Received:2010-06-27 Online:2010-10-15 Published:2010-12-29
Contact: ZHANG Xue-cheng2 E-mail:xczhang@ouc.edu.cn

Abstract

Abstract:

A full length cDNA of squalene synthase (SQS) gene was isolated from Schizochytrium limacinum using homologybased cloning and RACE methods. Molecular biological analysis showed that the full length cDNA was of 1672 bp, with a 1338 bp open reading frame encoding 446 amino acids, and the SQS gene was a single copy gene in Schizochytrium limacinum. Homology analysis showed that Schizochytrium limacinum SQS cDNA had five consensus regions. The SQS cDNA was constructed into the prokaryotic expression vector pGEX-4T-3 and expressed in E.coli BL21. SDS-PAGE and Western blotting analyses showed that the recombinant protein was mainly in the form of inclusion body, of which molecular weight was as expected, and had good immunogenicity.

Key words: Schizochytrium limacinum, squalene synthase, cDNA cloning, prokaryotic expression

CLC Number:

Q786

ZHU Lu-ying1,2，ZHU Qing-hua3,2，ZHANG Xue-cheng2,*. Cloning and Prokaryotic Expression of Squalene Synthase Gene from Schizochytrium limacinum[J]. FOOD SCIENCE, 2010, 31(19): 263-267.

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Cloning and Prokaryotic Expression of Squalene Synthase Gene from Schizochytrium limacinum

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